Method of Analysis of Herbs - III

Chinese name

Herb name

Method of Analysis

Key ingredients

Assay

虎杖

Huzhang

Polygoni Cuspidati rhizoma et radix

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:254nm,28.0℃, 1ml/min;Mobile phase:   Methanol:0.1%Phosphoric acid =80:20

Emodin

0.15%

虎杖

Huzhang

Polygoni Cuspidati rhizoma et radix

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:306nm,28.0℃, 1ml/min;Mobile phase:   Acetonitrile:Water=23:77

Polydatin

1.30%

罗布麻叶

Luobumaye

Apocyni Veneti Folium

Aglient Zorbax Eclipse Plus C18,150mm x 4.6mm ,5μm;10μl, λ:256nm,30.0℃, 1ml/min;Mobile phase:   Acetonitrile: 0.2%Phosphoric acid =15:85

Hyperoside

4.10%

垂盆草

Chuipencao

Sedi Herba

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm

20μl, λ:360nm,30.0℃,   1ml/minMobile phase: Methanol:0.4%Phosphoric acid =45:55

Isorhamnetin

Kaempferide

Quercetin

0.009%

0.006%

0.041%

侧柏叶

Cebaiye

Platycladi Cacumen

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:254nm,30.0℃, 1ml/min;Mobile phase: Methanol:0.01mol/L   KH2PO4: Glacial Acetic acid=40:60:1.5

Quercitrin

0.36%

金果榄

Jinguolan

Tinosporae Radix

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm,10μl, λ:210nm,25.0℃, 1ml/min;Mobile phase:   Acetonitrile:Water=35:65

Columbin

1.10%

金钱草

Jinqiaocao

Lysimachiae Herba

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:360nm,25.3℃, 1ml/min;Mobile phase: Methanol:   0.5%Phosphoric acid=50:50

Kaempferide,Quercetin

0.078%

金银花

Jinyinhua

Lonicerae Japonicae Flos

Aglient Zorbax Eclipse Plus C18,250mm x 4.6mm ,5μm,20μl, λ:327nm,40.0℃, 1ml/min;Mobile phase: A:0.4%Phosphoric   acid ;B:Acetonitrile;B:0-7min 10%,7-8min 10%-60%, 8-12min 60%,12-13min   60%-10%

Chlorogenic acid

3.56%

泽泻

Zexie

Alismatis Rhizoma

Aglient Extend C18, 250mm x 4.6mm ,5μm,20μl, λ:208nm,25.0℃, 1ml/min;Mobile phase:   Acetonitrile:Water=73:27

Alisol B 23-acetate

0.12%

贯叶金丝桃

Guanyejinsitao

Hyperici Perforati Herba

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm,20μl, λ:360nm,26.2℃, 1ml/min;Mobile phase: Acetonitrile:0.1%Phosphoric   acid =16:84

Hyperin

0.46%

荆芥

Jingjie

Schizonepetae Herba

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:252nm,27.6℃, 1ml/min;Mobile phase: Methanol: Water=80:20

Pulegone

0.056%

茵陈

Yinchen

Artemisiae Scopariae Herba

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:327nm,25.0℃, 1ml/min;Mobile   phase:Acetonitrile:0.05%Phosphoric acid =10:90

Chlorogenic Acid

0.73%

葫芦巴

Huluba

Trigonellae Semen

Aglient Eclipse Plus C18,250mm x 4.6mm ,5μm

20μl, λ:265nm,25.0℃,   1ml/min;Mobile phase: Methanol:0.05% DDBS: Glacial acetic acid=20:80:0.1

Trigonelline

0.33%

胡黄连

Huhuanglian

Picrorhizae Rhizoma

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,20μl, λ:275nm,28.0℃, 1ml/min;Mobile phase:Methanol:Water:Phosphoric   acid =35:65:0.1

Amphicoside I,Amphicoside II

5.45%

胡椒

Hujiao

Piperis Fructus

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:343nm,25.0℃, 1ml/min;Mobile phase:   Methanol:Water=65:35

Piperine

4.24%

枳壳

Zhiqiao

Aurantii Fructus

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:283nm,25.0℃, 1ml/min;Mobile phase:   Acetonitrile:Water=20:80

Naringin

Neohesperidin

5.18%

4.24%

枳实

Zhishi

Aurantii Fructus Immaturus

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm,20μl, λ:275nm,27.3℃, 1ml/min;Mobile phase: Methanol: KH2PO4(DDBS, Glacial acetic acid) =50:50

Synephrine

0.3%

栀子

Zhizi

Gardeniae Fructus

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm;20μl, λ:238nm,28.0℃, 1ml/min;Mobile phase:   Acetonitrile:Water=15:85

Geniposide

4.50%

厚朴

Houpu

Magnoliae Officinalis Cortex

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm;10μl, λ:294nm,28.0℃, 1ml/min;Mobile phase:   Methanol:Water=78:22

Magnolol

Honokiol

0.11%

0.05%

骨碎补

Gusuibu

Drynariae Rhizoma

Aglient Eclipse Plus C18,250mm x 4.6mm ,5μm,20μl, λ:283nm,27.4℃, 1ml/min;Mobile phase: Methanol: Acetic acid:Water =35:4:65

Naringin

0.54%

香加皮

Xiangjiapi

Periplocae Cortex

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:278nm,25.0℃, 1ml/min;Mobile phase:   Methanol:Water:Acetic acid   =70:30:0.2

4-Methoxysalicylaldehyde

0.017%

香薷

Xiangru

Moslae Herba

HP-Innowax,30m   x 0.25mm ,0.25μm;1μl, Inlet:250℃,Detector:260℃,Column:190℃.FID

Thymol,Carvacrol

0.46%

姜黄

Jianghuang

Curcumae Longae Rhizoma

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:430nm,28.0℃, 1ml/min,Mobile phase:   Acetonitrile:1% Glacial  acetic acid solution=48:52

Curcumin

2.6%

前胡

Qianhu

Peucedani Radix

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm,10μl, λ:321nm,25.0℃, 1ml/min;Mobile phase: Methanol: Water =75:25

Praeruptorin A

Paeruptorin B

2.69%

0.29%

洋金花

Yangjinhua

Daturae Flos

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm,40μl, λ:216nm,28.0℃, 1ml/min;Mobile phase:   Acetonitrile :0.07%Na3PO4 solution(contain 0.0175mol/L DDBS ,PH6.0) =50:100

Scopolamine,Hydrobromide

0.021%

穿山龙

Chuanshanlong

Dioscoreae Nipponicae Rhizoma

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:203nm,25.0℃, 1ml/min,Mobile phase:   Acetonitrile:Water=55:45

Dioscin

1.97%

穿心莲

Chuanxinlian

Andrographis Herba

Aglient Extend C18,150mm x 4.6mm ,5μm,20μl, λ:225nm,27.9℃, 1ml/min,Mobile phase:   Methanol:Water=52:48

Andrographolide

0.48%

秦皮

Qinpi

Fraxini Cortex

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:334nm,30.0℃, 1ml/min,Mobile phase:   Acetonitrile:0.1% Phosphoric acid=8:92

Esculin

Aesculetin

3.30%

0.18%

莱菔子

Laifuzi

Raphani Semen

Aglient Zorbax phenyl,150mm x 4.6mm ,5μm,20μl, λ:326nm,30.0℃, 1ml/min,Mobile phase: Acetonitrile:3% Glacial acetic acid=15:85

Sinapine thiocyanate

98.00%

荷叶

Heye

Nelumbinis Folium

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm;20μl, λ:270nm,25.0℃, 1ml/min;Mobile phase:   Acetonitrile:Water:triethylamine:Acetic acid solution=27:70.6:1.6:0.78

Nuciferine

0.09%

桂枝

Guizhi

Cinnamomi Ramulus

Aglient Zorbax Eclipse Plus C18,250mm x 4.6mm ,5μm;20μl, λ:290nm,25.0℃, 1ml/min;Mobile phase:   Acetonitrile:Water=32:68

Cinnamic aldehyde

7.70%

桃仁

Taoren

Peach Seed

Aglient Zorbax XDS C18;150mm x 4.6mm ,5μm;20μl, λ:210nm,30.0℃, 1ml/min;Mobile phase:   Methanol:Water=20:80

Amygdalin

3.19%

夏天无

Xiatianwu

Corydalis Decumbentis Rhizoma

Aglient Extend C18,250mm x 4.6mm ,5μm;20μl, λ:289nm,25.0℃, 1ml/min;Mobile phase:   Acetonitrile : TEAA =18:82

Protopine

0.41%

夏天无

Xiatianwu

Corydalis Decumbentis Rhizoma

Aglient Extend C18,250mm x 4.6mm ,5μm;20μl, λ:345nm,25.0℃, 1ml/min;Mobile phase:   Acetonitrile : TEAA =18:82

Palmatine chloride

0.22%

夏枯草

Xiakucao

Prunellae Spica

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,5μl, λ:330nm,25.0℃, 1ml/min;Mobile phase: Methanol:0.1% trifluoroacetic acid=42:58

Rosmarinic acid

1.40%

柴胡

Chaihu

Bupleuri Radix

Aglient Zorbax Eclipse Plus C18;250mm x 4.6mm ,5μm20μl, λ:210nm,25.0℃, 1ml/min;Mobile phase: A:Acetonitrile,B:Water,A:0-50min   25-90%;50-55min 90%;55-56min 90-25%

Saikosaponin a

Saikosaponin d

0.55%

0.45%

积雪草

Jixuecao

Centellae Herba

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,20μl, λ:205nm,30.0℃, 1ml/min;Mobile phase:   Acetonitrile:Beta-cyclodextrin solution=24:76

Asiaticoside

Madecassoside

1.52%

1.37%

射干

Shegan

Belamcandae Rhizoma

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm;20μl, λ:266nm,28.0℃, 1ml/min;Mobile phase: Methanol:0.2% Phosphate acid=53:47

Irisflorentin

0.21%

徐长卿

Xuchangqing

Cynanchi Paniculati Radix Et Rhizoma

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,20μl, λ:274nm,25.0℃, 1ml/min;Mobile phase: Methanol:Water=45:55

Paeonol

0.97%

高良姜

Gaoliangjiang

Alpiniae Officinarum Rhizoma

Aglient Zorbax XDB-C18,150mm x 4.6mm ,5μm,20μl, λ:266nm,25.0℃, 1ml/min;Mobile phase: Methanol:0.2% Phosphate acid=63:37

Galangin

4.74%

粉葛

Fenge

Puerariae Thomsonii Radix

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm,20μl, λ:250nm,26.1℃, 1ml/min;Mobile phase:   Methanol:Water=25:75

Puerarin

0.81%

益母草

Yimucao

Leonuri Herba

赛分 Polar-Imidazole,250mm x 4.6mm   ,5μm;20μl,  Atomization temperature:   70.0℃, column temperature: 25.0℃ ,1ml/min;Mobile   phase: Acetonitrile: 0.2%Acetic   acid=80:20

Stachydrine ,ydrochloride

0.98%

浙贝母

Zhebeimu

Fritillariae Thunbergii Bulbus

Aglient Zorbax SB C18;250mm x 4.6mm ,5μm,30μl, Atomization temperature: 50.0℃, 1ml/min;Mobile phase: Acetonitrile:Water: triethylamine=70:30:0.3

Peimine,Peiminine

0.03%

娑罗子

Suoluozi

Aesculi Semen

Aglient Eclipse Plus C18;250mm x 4.6mm ,5μm;20μl, λ:220nm,26.8℃, 1ml/min;Mobile phase:   Acetonitrile:0.2%Phosphoric acid =36:64

Sodium Aescinate

0.32%

桑叶

Sangye

Mori Folium

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm;10μl, λ:358nm,29.3℃, 1ml/min;Mobile phase: A:Methanol,B:0.5%Phosphoric   acid ;A:0-5min 30%,5-10min 30-35%,10-15min 35-40%,15-18min 40-50%

Rutin

0.14%

黄芩

Huangqin

Scutellariae Radix

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm;20μl, λ:280nm,27.3℃, 1ml/min;Mobile phase:   Methanol:Water:Phosphoric acid=47:53:0.2

Baicalin

13.89%

黄连

Huanglian

Coptidis Rhizoma

Aglient Extend C18,250mm x 4.6mm ,5μm,10μl, λ:345nm,25.0℃, 1ml/min;Mobile phase:   Acetonitrile:0.05mol/l Potassium dihydrogen phosphate=50:50

Epiberberine

Palmatine

Coptisine

Berberine

1.29%

2.48%

2.48%

9.17%

黄藤

Huangteng

Fibraureae Caulis

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm;20μl, λ:345nm,40.0℃, 1ml/min;Mobile phase: 0.4%Phosphoric   acid: Acetonitrile =68:32

Palmatine chloride

7.24%

菊花

Juhua

Chrysanthemi Flos

Aglient Zorbax Eclipse Plus C18,250mm x 4.6mm ,5μm,5μl, λ:348nm,25.0℃, 1ml/min;Mobile phase:   A:0.1%Phosphoric acid solution;B:Methanol:Acetonitrile(60:40%);B :0-7min   10-25%,7-27min 25-27%,27-40min 27%,40-42min 27-100%,42-45min 100%,45-45.5min   100%-25%

Chlorogenic acid

Galuteolin

(-)-3,5-Dicaffeoyl quinic acid

0.51%

0.94%

1.88%

救必应

Jiubiying

Ilicis Rotundae Cortex

Aglient Zorbax Eclipse Plus C18,250mm x 4.6mm ,5μm,5μl, λ:210nm,25.0℃, 1ml/min;Mobile phase: A:Water;B:   Acetonitrile;B :0-10min 10%,10-20min 10-40%,20-30min 40%,30-31min 40-10%,31-40min   10%

Syringin

Pedunculoside

1.71%

6.90%

蛇床子

Shechuangzi

Cnidii Fructus

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:322nm,28.0℃, 1ml/min;Mobile phase:   Methanol:Water:65:35

Osthole

1.42%

银杏叶

Yinxinye

Ginkgo Folium

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:360nm,28.7℃, 1ml/min;Mobile phase:   Methanol:0.4%Phosphoric acid =50:50

Quercetin,Kaempferol,Isorhamnetin

0.56%

银杏叶

Yinxinye

Ginkgo Folium

Aglient Extend-C18, 250mm x 4.6mm ,5μm,5μl, Atomization   temperature:50℃,1ml/min;Mobile   phase: Methanol:Tetrahydrofuran:Water=25:10:65

Bilobalide,Terpene Lactones A,Terpene Lactones   B,Terpene Lactones C

1.62%

淫羊藿

Yinyanghuo

Epimedii Folium

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:270nm,27.0℃, 1ml/min;Mobile phase:   Acetonitrile:Water=30:70

Icariin

0.07%

密蒙花

Mimenghua

Buddlejae Flos

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:326nm,28.0℃, 1ml/min;Mobile phase:   Methanol:Water: Acetic acid =45:54.5:0.5

Linarin

9.08%

续断

Xuduan

Dipsaci Radix

Aglient Eclipse Plus C18,250mm x 4.6mm ,5μm,20μl, λ:212nm,30.6℃, 1ml/min;Mobile phase: Acetonitrile:Water=30:70

Asperosaponin Ⅵ

4.11%

款冬花

Kuandonghua

Farfarae Flos

Aglient Extend C18, 250mm x 4.6mm ,5μm;20μl, λ:220nm,25.0℃, 1ml/min;Mobile phase:   Methanol:Water=80:20

Tussilagone

0.14%

葛根

Gegen

Puerariae Lobatae Radix

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm;20μl, λ:250nm,26.0℃, 1ml/min;Mobile phase:Methanol:Water=25:75

Puerarin

0.4%

紫花前胡

Zihuaqianhu

Peucedani Decursivi Radix

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm;5μl, λ:334nm,25.0℃, 1ml/min;Mobile   phase:Methanol:Water=36:64

Nodakenin

0.99%

紫草

Zicao

Arnebiae Radix

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:275nm,27.6℃, 1ml/min;Mobile phase:   Acetonitrile:Water: Formic acid=70:30:0.005

β,β-dimethyl-acry-lalkannin

0.36%

蓝布正

Lanbuzheng

Gei Herba

Aglient Zorbax SB-Aq C18,250mm x 4.6mm ,5μm;20μl, λ:273nm,30.0℃, 1ml/min;Mobile phase: Methanol: Phosphoric acid=7:93

Gallic acid

8.61%

蒲公英

Pugongying

Taraxaci Herba

Aglient Eclipse Plus C18,250mm x 4.6mm ,5μm,20μl, λ:323nm,27.0℃, 1ml/min;Mobile   phase:Methanol:Phosphate buffer solution=23:77

Caffeic acid

0.97%

蒲黄

Puhuang

Typhae Pollen

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,20μl, λ:254nm,30.0℃, 1ml/min;Mobile phase:   Acetonitrile:0.05% Phosphoric acid =15:85

Isorhamnetin-3-O-neohespeidoside

Typhaneoside

0.44%

0.51%

槐花

Huaihua

Sophorae Flos

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:257nm,28.8℃, 1ml/min,Mobile phase:   Methanol:1%Acetic acid =32:68

Rutin

7.34%

槐角

Huaijiao

Sorphorae Fructus

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,10μl, λ:260nm,28.0℃, 1ml/min;Mobile phase: Methanol:   Acetonitrile: 0.07% Phosphoric acid =12:20:68

Sophoricoside

5.94%

路路通

Lulutong

Liquidambaris Fructus

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,5μl, atomization   temperature:50.0℃,   1ml/min,Mobile phase: Methanol: Methanol:Water: Acetic acid=87:13:0.1

Betulonicacid

0.55%

蜂胶

Fengjiao

Propolis

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm;10μl, λ:268nm,28.0℃, 1ml/min;Mobile phase:   Methanol:0.15%Phosphoric acid =64:36

Chrysin

Galangin

0.40%

0.70%

锦灯笼

Jindenglong

Physails Calyx Seu Fructus

Aglient Zorbax plus-C18,150mm x 4.6mm ,5μm,20μl, λ:350nm,30.0℃, 1ml/min;Mobile phase: Acetonitrile:   0.2% Phosphoric acid =20:80

Luteoloside

0.21%

矮地茶

Aidicha

Ardisiae Japoncae Herba

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,20μl, λ:275nm,28.0℃, 1ml/min,Mobile phase: Methanol:Water   =20:80

Bergenin

1.40%

蔓荆子

Manjingzi

Viticis Fructus

Aglient Eclipse Plus,150mm x 4.6mm ,5μm,20μl, λ:258nm,26.3℃, 1ml/min,Mobile phase: Methanol:   0.4% Phosphoric acid =60:40

Casticin

0.18%

槟榔

Binglang

Arecae Semen

Sepax HP-SCX,250mm x 4.6mm ,5μm,10μl, λ:215nm,40.0℃, 0.5ml/min;Mobile phase:   Acetonitrile:Phosphoric acid =62:38

Arecoline ,hydrobromide

0.21%

漏芦

Loulu

Rhapontici Radix

Aglient Zorbax plus C18,250mm x 4.6mm ,5μm,10μl, λ:247nm,30.0℃, 1ml/min;Mobile phase: Methanol:Water   =31:69

Beta ecdysterone

0.09%

墨旱莲

Mohanlian

Ecliptae Herba

Aglient Zorbax plus C18,250mm x 4.6mm ,5μm,10μl, λ:351nm,40.0℃, 1ml/min,Mobile phase: A:0.5% Acetic acid,B: Acetonitrile;B:0-10min   35-59%;10-20min,59%;20-21min,59-95%;21-26min,95%;26-27min,95%-35%

Wedelolactone

0.24%

薏苡仁

Yiyiren

Coicis Semen

Aglient Eclipse plus C18,150mm x 4.6mm ,5μm,10μl, atomization   temperature:75.0℃,   1ml/min;Mobile phase: Acetonitrile: Dichloromethane=65:35

Glycerol Trioleate

0.61%

橘红

Juhong

Rubrum Exocarpium Citri

Aglient Eclipse plus,150mm x 4.6mm ,5μm,20μl, λ:284nm,27.3℃, 1ml/min;Mobile phase: Methanol:Water   =40:60

Hesperidin

0.26%

藁本

Gaoben

Ligustici Rhizoma Et Radix

Aglient Eclipse plus,250mm x 4.6mm ,5μm,20μl, λ:320nm,25.9℃, 1ml/min;Mobile phase: Methanol:Water   =38:62

Ferulic Acid

0.21%

蟾酥

Chansu

Bufonis Venenum

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,20μl, λ:296nm,29.7℃, 1ml/min,Mobile phase: 0.5% KH2PO4: Acetonitrile=50:50

Resibufogenin

Cinobufagin

3.41%

0.11%

麝香

Shexiang

Moschus

DB-1701P 30mx0.32mmx0.25μm;1μl,Column   temperature:200℃, 1ml/min

Muscone

0.19%