Method of Analysis of Herbs - I

Chinese name

Herb name

Method of Analysis

Key ingredients

Assay

丁公藤

Dinggongteng

Erycibes Caulis

Aglient Bonus RP C18,250mm x 4.6mm ,5μm,20μl, λ:298nm,27.0℃, 1ml/min;Mobile   phase:Methanol:Water:Glacial acetic acid=32:68:0.16

Scopoletin

0.045%

丁香

Dingxiang

Caryophylli Flos

HP-Innowax,30m x 0.25mm ,0.25μm,1μl, Inlet temperature: 250℃, Detector temperature: 260℃, Column Temperature: 190℃. Detector:FID

Eugenol

10.46%

人参

Renshen

Ginseng Radix et rhizoma

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:203nm,30.0℃, 1ml/min;Mobile phase:A:Acetonitrile, B:Water,   A:0-35min 19%,35-55min 19-29%,55-70min 29%,70-100min 29%-40%

Ginsenosides (Rg1,Re,Rb1)

0.22%

人参叶

Renshenye

Ginseng Folium

Aglient Eclipse plus C18,150mm x 4.6mm ,5μm,20μl, λ:203nm,25.0℃, 1ml/min;Mobile   phase:Acetonitrile:0.05%Phosphoric acid =1:4

Ginsenosides (Rg1,Re)

3.50%

儿茶

Ercha

Catechu

Aglient Eclipse plus C18,150mm x 4.6mm ,5μm,20μl, λ:280nm,35.0℃, 1ml/min;Mobile   phase:Citric acid solution:DMF:Tetrahydrofuran=82:14.5:3.5

Catechin Epicatechin

30.37%

三七

Sanqi

Notoginseng radix et rhizoma

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:203nm,25.0℃, 1ml/min;Mobile   phase: A:Acetonitrile, B:Water;A:0-12min 19%,12-60min 19-36%

Notoginsengde R1,Ginsenosides   (Rg1,Rb1)

9.31%

三白草

Sanbaicao

Saururi Herba

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:230nm,30.0℃, 1ml/min;Mobile   phase: Methanol:Water=63:37

Sauchinone

0.18%

干姜

Ganjiang

Zingiberis Rhizoma

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:280nm,25.0℃, 1ml/min;Mobile   phase:Acetonitrile:Methanol:Water=40:5:55

6-Gingerol

0.28%

土贝母

Tubeimu

Bolbostematis Rhizoma

Aglient Extend C18,150mm x 4.6mm ,5μm,20μl, λ:214nm,28.0℃, 1ml/min;Mobile   phase: Methanol:Water=65:35

Tubeimoside

0.96%

土荆皮

Tujingpi

Pseudolaricis Cortex

Aglient Eclipse XDB-C18,250mm x 4.6mm ,5μm,20μl, λ:332nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile:0.5% Phosphoric acid =17:83

Pseudolaric acid

0.59%

大叶紫珠

Dayezizhu

Callicarpae Macrophyllae Folium

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:280nm,25.0℃, 1ml/min;Mobile   phase:Acetonitrile:Methanol:Water=40:5:55

Acteoside

0.58%

大青叶

Daqingye

Isatidis Folium

Aglient Extend C18,250mm x 4.6mm ,5μm,20μl, λ:289nm,28.0℃, 1ml/min;Mobile   phase: Methanol:Water=75:25

Indirubin

0.009%

大黄

Dahuang

Rhei Radix Et Rhizoma

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:254nm,28.0℃, 1ml/min;Mobile   phase:Methanol:0.1% Phosphoric acid solution=70:30

Rhein,Chrysophanol,Emodin,Aloe   Emodin,Physcion

2.43%

大蓟

Daji

Cirsii Japonici Herba

Aglient Eclipse plus C18,250mm x 4.6mm ,5μm,20μl, λ:330nm,28.0℃, 1ml/min;Mobile   phase: Acetonitrile:0.1% Phosphoric acid =21:79

Pectolinarin

0.70%

山豆根

Shandougen

Sophorae Tonkinensis Radix Et   Rhizoma

Aglient Zorbax NH2,250mm x 4.6mm ,5μm,20μl, λ:210nm,25.0℃, 1ml/min;Mobile   phase:A: Acetonitrile:3% Phosphoric acid:   Ethanol=80:10:10,B:0.05mol/L NaH2PO4   Solution, A:B=95:5

Matrine

Oxymatrine

0.51%

1.06%

山茱萸

Shanzhuyu

Corni Fructus

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,10μl, λ:240nm,27.4℃, 1ml/min;Mobile   phase: Acetonitrile:Water=15:85

Loganin

0.79%

山银花

Shanyinhua

Lonicerae Flos

Aglient Zorbax Eclipse plus C18,250mm x 4.6mm ,5μm,10μl, λ:330nm,20.0℃, 1ml/min;Mobile phase:A: 0.4% acetic acid   solution,B: Acetonitrile,B:0-20min,8%;20-25min,8-30%;25-37.5min30-35%;37.5-38.5min35-100%;38.5-43.5min   100%,43.5-44.5min 100%-8%

Chlorogenic   Acid

3.07%

山楂叶

Shanzhaye

Crataegi Folium

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:363nm,28.0℃, 1ml/min;Mobile   phase:Acetonitrile:Methanol:Tetrahydrofuran:0.5%Acetic acid =1:1:19.4:78.6

Hyperin

0.25%

千里光

Qianliguang

Senecionis Scandentis Herbra

Aglient Zorbax Eclipse plus C18,250mm x 4.6mm ,5μm,20μl, λ:360nm,15.0℃, 1ml/min;Mobile   phase: Acetonitrile:0.2%Acetic acid =18:82

Hyperin

0.10%

川牛膝

Chuanniuxi

Cyathulae Radix

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:243nm,25.0℃, 1ml/min;Mobile phase:A:Methanol,B:Water;A:0-25min   30%-40%, 25-32min 40%, 32-33min 40%-100%

Cyasterone

0.10%

川芎

Chuanxiong

Chuanxiong Rhizoma

Aglient Extend C18,250mm x 4.6mm ,5μm

20μl, λ:321nm,30.0℃, 1ml/min;Mobile   phase: Methanol:1%Acetic acid =30:70

Ferulic Acid

0.10%

川射干

Chuanshegan

Iridis Tectori Rhizoma

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:265nm,28.0℃, 1ml/min;Mobile   phase: Methanol:0.05mol/L KH2PO4 Solution(PH=3.0)=32:68

Tectoridin

4.74%

川楝子

Chuanlianzi

Toosendan Fructus

Aglient Zorbax SB-C18,100mm x 2.1mm ,3.5μm,2μl,   λ:m/z=573(+),30.0℃,   0.2ml/min;Mobile phase:Acetonitrile:0.01%   Formic acid=31:69

Toosendanin

0.05%

广枣

Guangzao

Choerospondiatis Fructus

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:270nm,30.0℃, 1ml/min;Mobile   phase:Methanol:Water: Glacial Acetic Acid=1:99:0.3

Gallic acid

0.026%

广藿香

Guanghuoxiang

Pogostemonis Herba

HP-5 30mx0.32mm,0.25μm,1μl;   Temperature:280℃

Patchouli alcohol

0.56%

小蓟

Xiaoji

Cirsii Herba

Aglient Extend C18,250mm x 4.6mm ,5μm,20μl, λ:326nm,30.0℃, 1ml/min;Mobile   phase:Methanol: 0.5%Acetic acid =50:50

Linarin

0.17%

马钱子

Maqianzi

Strychni Semen

Aglient Eclipse plus C18,150mm x 4.6mm ,5μm,20μl, λ:260nm,26.9℃, 1ml/min;Mobile   phase: Acetonitrile:mixture of 0.01mol/L C12H25―OSO3Na and 0.02mol/L   KH2PO4(PH2.8)=21:79

Strychnine

Vauquline

7.12%

5.26%

天山雪莲

Tianshanxuelian

Saussureae Involucratae Herba

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:340nm,30.0℃, 1ml/min;Mobile   phase:A:Methanol,B:0.4%Phosphoric acid;A:0-12min19%,12-18min 19-35%,18-25min   35%

Rutin

Chlorogenic acid

0.40%

0.28%

天麻

Tianma

Gastrodiae Rhizoma

Aglient Zorbax SB-Aq,250mm x 4.6mm ,5μm,10μl, λ:220nm,28.0℃, 1ml/min;Mobile   phase:Acetonitrile:0.05%Phosphoric acid =3:97

Gastrodin

0.37%

木香

Muxiang

Aucklandiae Radix

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,40μl, λ:225nm,27.0℃, 1ml/min;Mobile   phase:Methanol:Water=65:35

Costunolide

Dehydrocostunolide

2.96%

1.57%

木贼

Muzei

Equiseti Hiemalis Herba

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:365nm,26.2℃, 1ml/min;Mobile   phase:Acetonitrile:0.4%Phosphoric acid=50:50

Kaempferol

0.02%

木通

Mutong

Akebiae Caulis

Aglient Zorbax SB-C18,150mm x 4.6mm ,5μm,5μl, λ:330nm,25.0℃, 1ml/min;Mobile   phase:Methanol:Wate:Phosphoric acid=35:65::0.5

Calceolarioside B

2.09%

五味子

Wuweizi

Schisandrae chinensis fructus

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm,20μl, λ:250nm,28.0℃, 1ml/min;Mobile   phase: Methanol:Water=65:35

Schisandrin

0.38%

五倍子

Wubeizi

Galla Chinensis

Aglient Eclipse Plus,150mm x 4.6mm ,5μm,20μl, λ:273nm,26.5℃, 1ml/min;Mobile   phase:Methanol:0.1%Phosphoric acid =15:85

Gallic acid

60.33%

瓦松

Wasong

Orostachydis Fimbriati Herba

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,10μl, λ:360nm,28.0℃, 1ml/min;Mobile   phase:Methanol:0.5%Phosphoric acid=47:53

Quercetin,Kaempferol

0.082%

牛蒡子

Niubangzi

Arctii Fructus

Aglient Bonus-RP C18,250mm x 4.6mm ,5μm,20μl, λ:280nm,25.6℃, 1ml/min;Mobile   phase:Methanol:Water=48:52

Arctiin

5.92%

牛膝

Niuxi

Achyranthis Bidentatae Radix

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,20μl, λ:250nm,30.0℃, 1ml/min;Mobile   phase:Acetonitrile:Water:Formic acid =16:84:0.1

Ecdysterone

0.036%

升麻

Shengma

Cimicifugae Rhizoma

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,40μl, λ:316nm,28.0℃, 1ml/min;Mobile   phase:Acetonitrile:0.1% Phosphoric acid =13:87

Isoferulic acid

0.17%

化橘红

Huajuhong

Citri Grandis Exocarpium

Aglient Zorbax SB-Aq, 250mm x 4.6mm ,5μm,20μl, λ:283nm,26.9℃, 1ml/min;Mobile   phase:Methanol:Acetic acid:Water=32:4:64

Naringin

1.80%

丹参

Danshen

Salviae Miltiorrhizae Radix et   rhizome

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,10μl, λ:286nm,28.0℃, 1ml/min;Mobile   phase:Methanol:Acetonitrile:Formic acid:Water=30:10:1:59

Salvianolic acid B

6.60%

丹参

Danshen

Salviae Miltiorrhizae Radix et   rhizoma

Aglient Eclipse plus C18,250mm x 4.6mm ,5μm,20μl, λ:270nm,26.4℃, 1ml/min;Mobile   phase:Methanol:Water=75:25

Tanshinone II A

0.58%

水飞蓟

Shuifeiji

Silybi Fructus

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,20μl, λ:287nm,30.0℃, 1ml/min;Mobile   phase:Methanol:Water=42:58

Silybin 1,Silybin 2

2.96%

功劳木

Gonglaomu

Mahoniae Caulis

Aglient Extend C18,250mm x 4.6mm ,5μm,20μl, λ:265nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile: 0.05mol/L KH2PO4 (PH 3.0)=30:70

Berberine hydrochloride

Palmatine hydrochloride

0.31%

1.28%

甘草

Gancao

Glycyrrhizae Radix et rhizome

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,10μl, λ:237nm,60.0℃, 1ml/min;Mobile   phase: A:0.05%Phosphoric acid solution,

B:Acetonitrile;B: 0-8min   19%,8-60min 19-35%,60-70min 35-40%,70-75min 40-100%,75-80min 100-19%

Glycyrrhizic acid,Liquiritin

2.32%

1.22%

石韦

Shiwei

Pyrrosiae Folium

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,10μl, λ:326nm,26.1℃, 1ml/min;Mobile   phase: Acetonitrile:0.5%Phosphoric acid=11:89

Chlorogenic acid

0.35%

布渣叶

Buzhaye

Microctis Folium

Aglient Extend C18,250mm x 4.6mm ,5μm,10μl, λ:339nm,25.0℃, 1ml/min;Mobile   phase:Methanol: 0.4%Phosphoric acid=25:75

Vitexin

0.11%

龙胆

Longdan

Gentianae Radix Et Rhizoma

Aglient Zorbax Eclipse plus C18,250mm x 4.6mm ,5μm,10μl, λ:270nm,30.0℃, 1ml/min;Mobile   phase: Methanol:Water=25:75

Gentiopicroside

3.30%

仙茅

Xianmao

Curculiginis Rhizoma

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,20μl, λ:285nm,25.0℃, 1ml/min;Mobile   phase:Acetonitrile:0.1%Phosphoric acid=21:79

Curculigoside

0.13%

白芍

Baishao

Paeoniae Radix Alba

Aglient Zorbax SB C18,250mm x 4.6mm ,5μm,20μl, λ:250nm,28.0℃, 1ml/min;Mobile   phase: Acetonitrile:0.1%Phosphoric acid =14:86

Paeoniflorin

3.49%

白芷

Baizhi

Angelicae Dahuricae Radix

Aglient Eclipse plus C18,150mm x 4.6mm ,5μm,20μl, λ:300nm,28.0℃, 1ml/min;Mobile   phase: Methanol:Water=65:35

Imperatorin

0.083%

白屈菜

Baiqucai

Chelidonii Herba

Aglient Eclipse plus C18,150mm x 4.6mm ,5μm,10μl, λ:269nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile:1% Triethylamine(PH3.0)=26:74

Chelerythrine

0.28%

白鲜皮

Baixianpi

Dictamni Cortex

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,10μl, λ:236nm,25.0℃, 1ml/min;Mobile   phase: Methanol:Water=60:40

Fraxinellone

Obacunone

0.30%

0.33%

瓜蒌子

Gualouzi

Trichosanthis semen tostum

Aglient plus C18,150mm x 4.6mm ,5μm,20μl, λ:230nm,30.0℃, 1ml/min;Mobile   phase: Methanol:Water=93:7

3,29-Dibenzoyl rarounitriol

0.099%

冬虫夏草

Dongchongxiacao

Cordyceps

Aglient Zorbax bonvs RP,250mm x 4.6mm ,5μm,20μl, λ:260nm,28.0℃, 1ml/min;Mobile   phase:Phosphate buffer solution(PH=6.5):Methanol=85:15

Adenosine

0.011%

冬凌草

Donglingcao

Rabdosiae Rubescentis Herba

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,20μl, λ:239nm,25.0℃, 1ml/min;Mobile   phase: Methanol:Water=55:45

Oridonin

0.25%

半枝莲

Banzhilian

Scutellariae Barbatae Herba

Aglient Eclipse plus C18,250mm x 4.6mm ,5μm,20μl, λ:335nm,30.6℃, 1ml/min;Mobile phase: Methanol:Water:Acetic acid=35:61:4

Scutellarin

0.39%

母丁香

Mudingxiang

Caryophylli Fructus

Aglient Extend C18,150mm x 4.6mm ,5μm,20μl, λ:280nm,25.2℃, 1ml/min;Mobile phase: Methanol:Water=65:35

Eugenol

0.56%

地肤子

Difuzi

Kochiae Fructus

Aglient Zorbax Eclipse plus C18,250mm x 4.6mm ,5μm,20μl, 1ml/min;The   atomization temperature:50℃;Mobile phase:Methanol:Water: Glacial acetic   acid=85:15:0.2

Ic Momordin Ic

1.80%

地黄

Dihuang

Rehmanniae Radix

Aglient Zorbax SB Aq,250mm x 4.6mm ,5μm,20μl, λ:210nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile:0.1%Phosphoric acid =1:99

Catalpol

0.66%

地榆

Diyu

Sanguisorbae Radix

Aglient Zorbax SB Aq C18,250mm x 4.6mm ,5μm,20μl, λ:272nm,30.0℃, 1ml/min;Mobile   phase: Methanol:0.05%Phosphoric acid =5:95

Gallic acid

0.49%

地锦草

Dijincao

Euphorbiae Humifusae Herba

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,10μl, λ:360nm,25.3℃, 1ml/min;Mobile phase: Methanol:0.4%Phosphoric acid =50:50

Quercetin

0.11%

西红花

Xihonghua

Croci Stigma

Aglient Eclipse Plus C18,150mm x 4.6mm ,5μm,20μl, λ:440nm,28.0℃, 1ml/min;Mobile   phase: Methanol:Water=45:55

Crocin I,Crocin II

8.69%

西洋参

Xiyangshen

Panacis Quinquefolii Radix

Aglient Zorbax SB C18,150mm x 4.6mm ,5μm,10μl, λ:203nm,30.0℃, 1ml/min;Mobile phase: A:   Acetonitrile,B:0.1%Phosphoric acid;A: 0-25min 19-20%;25-60min,   20-40%;60-90min,40-55%;90-100min,55-60%

Ginsenoside

(Rg1,Re,Rb1)

0.13%

当归

Danggui

Angelicae sinensis radix

Aglient Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:316nm,28.0℃, 1ml/min;Mobile   phase: Acetonitrile:Water=17:83

Ferulic acid

0.087%